Özge Sezin SOMUNCU
(Assistant Professor) Bahçeşehir University, Faculty of Medicine
Title: Exosomes of Human Melanoma Cells Induce Cancer Development in Human Dermal Cells through Rab27a and c-Met
Biography
Biography: Özge Sezin SOMUNCU
Abstract
Exosomes are small membrane derived vesicles that transmit DNA constituents, mRNAs, microRNAs and proteins from donor cells to a receiver cell. Many divergent cells comprising mesenchymal cells, immune cells and cancer cells discharge exosomes. Studies show that cancer cell exosomes create the entry and reprogramming of essentials connected to tumor environment. A reference study established that melanoma-derived exosomes convey diverse proteins such as c-Met and Rab27a, which indicate a melanoma mark. Increased Met expressions in serum exosomes have thought to be a predictor of disease progression. Meanwhile, Rab27a has identified to be important in exosome discharge. Decreased expressions of Rab27a in human melanoma cells determined to diminish exosome release. This project aims to examine the effects of downregulation and upregulation of Rab27a and c-Met in human melanocytes by utilizing the isolated exosomes from a malignant melanoma cell lineage. Throughout the analysis of cancer-like formation; different protocols covering gene transfections, flow cytometry analysis of the transfection efficiency, Annexin-V apoptosis assays, tube formation assays, ELISA assays and gene expression profiling were performed. According to the results, exosomes derived from cancer cells conveyed information to healthy melanocytes/keratinocytes and induced cellular reaction with Met and Rab27a overexpression, therefore silencing their genes may be a beneficial approach for future treatment possibilities. The developing molecular contextual of melanoma exosomes and their implications for improved management of melanoma patients can be an astonishing therapeutic methodology for future actions.